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cells expressing mito snap  (New England Biolabs)


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    Structured Review

    New England Biolabs cells expressing mito snap
    ( a ) Confocal image of GFP-actin (red) recruitment to elongated, <t>Mito-SNAP-labelled</t> mitochondria (blue) in a HeLa cell. Actin assembly is enriched at sites of ER tubule (DsRed2-ER, green) overlap with mitochondria. Arrows indicate regions of Actin/ER co-localization at prospective sites of mitochondrial fission. ( b ) Enlarged image of Box B (+4 min), demonstrating co-localization of actin and ER at the site of mitochondrial fission. ( c ) Line scan indicating overlapping peak actin and ER intensity at site of mitochondrial constriction in ( b ). ( d ) Three-dimensional rendering of F-actin (LifeAct-GFP) and ER-tubules (DsRed2-ER) assembled on a constricted HeLa cell mitochondrion (Mito-SNAP). Scale bars, 1 μm ( a ), 0.5 μm ( b ) and 0.75 μm per unit ( d ).
    Cells Expressing Mito Snap, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 335 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cells+expressing+mito+snap/SNAP-Cell+647-SiR/pmc05056443-178-0-11
    Average 97 stars, based on 335 article reviews
    cells expressing mito snap - by Bioz Stars, 2026-09
    97/100 stars

    Images

    1) Product Images from "Dynamic actin cycling through mitochondrial subpopulations locally regulates the fission–fusion balance within mitochondrial networks"

    Article Title: Dynamic actin cycling through mitochondrial subpopulations locally regulates the fission–fusion balance within mitochondrial networks

    Journal: Nature Communications

    doi: 10.1038/ncomms12886

    ( a ) Confocal image of GFP-actin (red) recruitment to elongated, Mito-SNAP-labelled mitochondria (blue) in a HeLa cell. Actin assembly is enriched at sites of ER tubule (DsRed2-ER, green) overlap with mitochondria. Arrows indicate regions of Actin/ER co-localization at prospective sites of mitochondrial fission. ( b ) Enlarged image of Box B (+4 min), demonstrating co-localization of actin and ER at the site of mitochondrial fission. ( c ) Line scan indicating overlapping peak actin and ER intensity at site of mitochondrial constriction in ( b ). ( d ) Three-dimensional rendering of F-actin (LifeAct-GFP) and ER-tubules (DsRed2-ER) assembled on a constricted HeLa cell mitochondrion (Mito-SNAP). Scale bars, 1 μm ( a ), 0.5 μm ( b ) and 0.75 μm per unit ( d ).
    Figure Legend Snippet: ( a ) Confocal image of GFP-actin (red) recruitment to elongated, Mito-SNAP-labelled mitochondria (blue) in a HeLa cell. Actin assembly is enriched at sites of ER tubule (DsRed2-ER, green) overlap with mitochondria. Arrows indicate regions of Actin/ER co-localization at prospective sites of mitochondrial fission. ( b ) Enlarged image of Box B (+4 min), demonstrating co-localization of actin and ER at the site of mitochondrial fission. ( c ) Line scan indicating overlapping peak actin and ER intensity at site of mitochondrial constriction in ( b ). ( d ) Three-dimensional rendering of F-actin (LifeAct-GFP) and ER-tubules (DsRed2-ER) assembled on a constricted HeLa cell mitochondrion (Mito-SNAP). Scale bars, 1 μm ( a ), 0.5 μm ( b ) and 0.75 μm per unit ( d ).

    Techniques Used:

    Related Articles

    Expressing:

    Article Title: Dynamic actin cycling through mitochondrial subpopulations locally regulates the fission–fusion balance within mitochondrial networks
    Article Snippet: Cells were incubated in 30 nM TMRE in complete DMEM (T-669, Molecular Probes) for 15 min and washed 2 × in DMEM before live-cell imaging. .. Cells expressing Mito-SNAP were incubated with 2.5 μM SNAP-cell 647-SiR (S9102S, NEB) for 30 min and washed 2 × before imaging. .. All images were acquired on a spinning-disk confocal (UltraVIEW VoX; Perkin Elmer) on a Nikon Eclipse Ti microscope using an Apochromat × 100 1.49 numerical aperture oil-immersion objective (Nikon) in a temperature-controlled chamber (37 °C).

    Incubation:

    Article Title: Dynamic actin cycling through mitochondrial subpopulations locally regulates the fission–fusion balance within mitochondrial networks
    Article Snippet: Cells were incubated in 30 nM TMRE in complete DMEM (T-669, Molecular Probes) for 15 min and washed 2 × in DMEM before live-cell imaging. .. Cells expressing Mito-SNAP were incubated with 2.5 μM SNAP-cell 647-SiR (S9102S, NEB) for 30 min and washed 2 × before imaging. .. All images were acquired on a spinning-disk confocal (UltraVIEW VoX; Perkin Elmer) on a Nikon Eclipse Ti microscope using an Apochromat × 100 1.49 numerical aperture oil-immersion objective (Nikon) in a temperature-controlled chamber (37 °C).

    Imaging:

    Article Title: Dynamic actin cycling through mitochondrial subpopulations locally regulates the fission–fusion balance within mitochondrial networks
    Article Snippet: Cells were incubated in 30 nM TMRE in complete DMEM (T-669, Molecular Probes) for 15 min and washed 2 × in DMEM before live-cell imaging. .. Cells expressing Mito-SNAP were incubated with 2.5 μM SNAP-cell 647-SiR (S9102S, NEB) for 30 min and washed 2 × before imaging. .. All images were acquired on a spinning-disk confocal (UltraVIEW VoX; Perkin Elmer) on a Nikon Eclipse Ti microscope using an Apochromat × 100 1.49 numerical aperture oil-immersion objective (Nikon) in a temperature-controlled chamber (37 °C).



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    cells expressing mito snap  (New England Biolabs)
    97
    New England Biolabs cells expressing mito snap
    ( a ) Confocal image of GFP-actin (red) recruitment to elongated, <t>Mito-SNAP-labelled</t> mitochondria (blue) in a HeLa cell. Actin assembly is enriched at sites of ER tubule (DsRed2-ER, green) overlap with mitochondria. Arrows indicate regions of Actin/ER co-localization at prospective sites of mitochondrial fission. ( b ) Enlarged image of Box B (+4 min), demonstrating co-localization of actin and ER at the site of mitochondrial fission. ( c ) Line scan indicating overlapping peak actin and ER intensity at site of mitochondrial constriction in ( b ). ( d ) Three-dimensional rendering of F-actin (LifeAct-GFP) and ER-tubules (DsRed2-ER) assembled on a constricted HeLa cell mitochondrion (Mito-SNAP). Scale bars, 1 μm ( a ), 0.5 μm ( b ) and 0.75 μm per unit ( d ).
    Cells Expressing Mito Snap, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cells+expressing+mito+snap/SNAP-Cell+647-SiR/pmc05056443-178-0-11
    Average 97 stars, based on 1 article reviews
    cells expressing mito snap - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    ( a ) Confocal image of GFP-actin (red) recruitment to elongated, Mito-SNAP-labelled mitochondria (blue) in a HeLa cell. Actin assembly is enriched at sites of ER tubule (DsRed2-ER, green) overlap with mitochondria. Arrows indicate regions of Actin/ER co-localization at prospective sites of mitochondrial fission. ( b ) Enlarged image of Box B (+4 min), demonstrating co-localization of actin and ER at the site of mitochondrial fission. ( c ) Line scan indicating overlapping peak actin and ER intensity at site of mitochondrial constriction in ( b ). ( d ) Three-dimensional rendering of F-actin (LifeAct-GFP) and ER-tubules (DsRed2-ER) assembled on a constricted HeLa cell mitochondrion (Mito-SNAP). Scale bars, 1 μm ( a ), 0.5 μm ( b ) and 0.75 μm per unit ( d ).

    Journal: Nature Communications

    Article Title: Dynamic actin cycling through mitochondrial subpopulations locally regulates the fission–fusion balance within mitochondrial networks

    doi: 10.1038/ncomms12886

    Figure Lengend Snippet: ( a ) Confocal image of GFP-actin (red) recruitment to elongated, Mito-SNAP-labelled mitochondria (blue) in a HeLa cell. Actin assembly is enriched at sites of ER tubule (DsRed2-ER, green) overlap with mitochondria. Arrows indicate regions of Actin/ER co-localization at prospective sites of mitochondrial fission. ( b ) Enlarged image of Box B (+4 min), demonstrating co-localization of actin and ER at the site of mitochondrial fission. ( c ) Line scan indicating overlapping peak actin and ER intensity at site of mitochondrial constriction in ( b ). ( d ) Three-dimensional rendering of F-actin (LifeAct-GFP) and ER-tubules (DsRed2-ER) assembled on a constricted HeLa cell mitochondrion (Mito-SNAP). Scale bars, 1 μm ( a ), 0.5 μm ( b ) and 0.75 μm per unit ( d ).

    Article Snippet: Cells expressing Mito-SNAP were incubated with 2.5 μM SNAP-cell 647-SiR (S9102S, NEB) for 30 min and washed 2 × before imaging.

    Techniques: